ADC TOXICITY ATLAS
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ARX-305

Investigational
ARX305; ARX 305; NCB-002
Sponsor
Ambrx, Inc. (now J&J); NovoCodex Biopharmaceuticals; Zhejiang Medicine Co.
Indication
Advanced CD70-positive solid tumors (renal cell carcinoma predominant; broader ADCdb program also lists AML/NHL)
Target family
TNF receptor superfamily
RP2D dose
Not yet determined (FIH dose-escalation 0.24-4.2 mg/kg IV Q3W; Bayesian Optimal Interval design; RP2D/MTD selection ongoing)
Q3W (every 3 weeks)RP2D
01
Multi-organ toxicity

Fingerprint & organ drill-down

Also reported
0%severity100%
0
Assessed · clear
true 0%
No data
never assessed
3 adverse-event terms

Ocular

Any-grade
46.3%
G3+
0%
RP2D
sagittal schematic · Unknown

Tissues shaded by reported adverse-event rate.

Cornea
AE
41.5%
Express.
0.82%
Limbus
AE
-
Express.
1%
Conjunctiva
AE
-
Express.
0.64%
Lens
AE
-
Express.
-
Retina / RPE
AE
-
Express.
-
0 nTPM
Off-target signature

41.5% corneal toxicity, yet the CD70 (CD27 ligand / CD27L / TNFSF7) target is detected in only 0.82% of central cornea - toxicity is not explained by target expression.

Dominant tissue
Cornea
Surface subtype
Corneal (off-target)
Reversibility
Unknown
Reported ocular events
Dry eye46.3%
G3+ 0%n=19
Vision blurred43.9%
G3+ 0%n=18
Keratopathy41.5%
G3+ 0%n=17
02
Construct

Molecular anatomy

Antibody
IgG1
Humanized
Linker
Non-cleavable
2
DAR
Payload
Microtubule / tubulin polymerization inhibitor (anti-mitotic)
Linker structureC7H17NO4
COCCOCCOCCON
Payload structureC39H65N5O8
CC[C@H](C)C([C@@H](CC(=O)N1CCC[C@H]1[C@@H]([C@@H](C)C(=O)N[C@@H](CC2=CC=CC=C2)C(=O)O)OC)OC)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC
03
Antibody

Antibody & Fc engineering

Antibody
Proprietary high-affinity humanized anti-CD70 monoclonal antibody
Isotype
IgG1
Origin
Humanized
Fc modifications
-
Glycoengineering
-
Effector silencing
-
FcγR binding
Retained
C1q binding
Retained
04
Linker & conjugation

Linker chemistry

Linker profile
Linker
Oxime-PEG3
Class
Non-cleavable
Cleavage
Non-cleavable
Attachment
Site-specific (enzymatic/non-natural AA)
Conjugation
Site-specific via genetically encoded non-natural amino acid para-acetyl-phenylalanine (pAF); stable oxime conjugation (Ambrx EuCODE / Engineered Precision Biologics platform)
Symmetry
Site-specific (paired)
DAR (mean)
2
DAR homogeneity
Homogeneous
Plasma t½
-
Cleavage trigger
None - non-cleavable (stable oxime / PEG3 linker); no protease/acid/disulfide-cleavable motif; payload released only via antibody lysosomal proteolytic catabolism
Release control
Unconditional
Hydrophilicity mask
Discrete-PEG-spacer
Formula
C7H17NO4
Linker MW
179.216 Da
Linker TPSA
62.94 Ų
Linker xLogP
-0.4437
ADCdb linker
LIN0BDQFG
In-vitro stability
-
05
Payload

Payload & physicochemistry

Payload profile
Payload
AS269 (Amberstatin 269) - monomethyl auristatin F (MMAF) derivative
Class
Auristatin
Mechanism
Microtubule / tubulin polymerization inhibitor (anti-mitotic)
Released catabolite
pAF-AS269 (para-acetyl-phenylalanyl-amberstatin-269); charged, membrane-impermeable MMAF-derivative amino-acid catabolite (non-cleavable linker)
Mechanistic subtype
Tubulin-auristatin
Stereochem / salt
-
Bystander
No
PAMPA rank
-
MW
731.97 Da
XLogP3
2.1
logD₇.₄
-
TPSA
167 Ų
pKa
-
Charge pH 7.4
0
H-bond donors
4
H-bond acceptors
9
IC50 (HCEC)
-
Formula
C39H65N5O8
PubChem CID
56841603
ADCdb payload
PAY0QLDVX
Bioactivity note
AS269 = PEG4-aminooxy-MMAF (Amberstatin; opadotin; CAS 1415246-35-3; PubChem CID 89283237; C47H82N6O12, MW 923.2). MMAF/AS269 is a potent antimitotic tubulin-polymerization inhibitor; the intact AS269 and its free warhead are membrane-impermeable, giving low cell-free/uptake-inde
06
Dosing & regimen

Dosing

RP2D dose
Not yet determined (FIH dose-escalation 0.24-4.2 mg/kg IV Q3W; Bayesian Optimal Interval design; RP2D/MTD selection ongoing)
Schedule
Q3W (every 3 weeks)
Route
IV
Fractionated
No
n at RP2D
-
Dose basis
TBW
Trial phase
Phase 1
Dose-OAE available
No
Tox summary basis
RP2D
07
Ocular & expression

Ocular profile & eye-tissue target expression

Target profile
OAE any-grade
46.3 %
OAE grade 3+
0 %
OAE data status
reported
Severity (weighted)
13.89
Keratopathy
41.5 %
Conjunctival
-
Dry eye
46.3 %
Blurred vision
43.9 %
Dominant tissue
Cornea
Surface subtype
Corneal (off-target)
Grading scale
CTCAE (unversioned)
Reversibility
Unknown
Target expression in eye tissues (HCA detection · HPA bulk)
Cornea (central)
0.82 %
Cornea (limbal)
1 %
Conjunctiva
0.64 %
RPE
-
Retina (HPA)
0 nTPM
Cross-trial comparability
Ascertainment: Monitoring unknownScale: CTCAE (unversioned)Denominator: RP2D⚠ OAE rate not directly comparable across trials
08
Identity & registry

Identifiers, registry & notes

ADC id
arx-305
Approval status
Investigational
Approval year
-
UniProt
P32970
ADCdb ADC
DRG0BWCTV
ADCdb antibody
ANI0XZPUK
ADCdb target
TAR0KPFBI
Primary source
ESMO 2025 abstr 959P (Ann Oncol 2025, S0923-7534(25)02448-2)
Aliases & development codes
ARX305; ARX 305; NCB-002
Notes
ARX-305 (ARX305; NCB-002), Ambrx/NovoCodex anti-CD70 MMAF-class ADC. Composition (ADCdb DRG0BWCTV + Ambrx AACR 2023 #6318 / ESMO 2023 71P): humanized anti-CD70 antibody (isotype not disclosed; likely IgG1 on Ambrx pAF platform but left blank), site-specifically conjugated at the non-natural amino acid para-acetyl-phenylalanine (pAF) via a stable oxime bond to a non-cleavable Oxime-PEG3 linker (ADCdb LIN0BDQFG), DAR 2 (homogeneous). Payload is AS269 (Amberstatin 269), a potent, membrane-impermeable MMAF (auristatin F) derivative; ADCdb maps the payload to MMAF (PAY0QLDVX). Released catabolite is pAF-AS269. Membrane-impermeable + charged free carboxylate => no bystander effect. LINKER PHYSCHEM CAVEAT: ADCdb's LIN0BDQFG "Oxime-PEG3" entry catalogs only the methoxy-PEG3-oxyamine fragment (C7H17NO4, MW 179.216, TPSA 62.94, xLogP -0.4437), representing the oxime-PEG linker chemistry, not the full linker-payload. ADCdb displayed the SMILES as "MOCCOCCOCCON" - corrected here to "COCCOCCOCCON" per the listed InChI (InChIKey UPZRLQLRXOQKOM; methoxy-triethyleneglycol-aminooxy). ADCdb cross-references the same linker to ARX-517. PAYLOAD PHYSCHEM: reported as MMAF standard values (PubChem CID 10395173): MW 731.97, formula C39H65N5O8, XLogP3 2.1, TPSA 167, formal charge 0 (zwitterionic at pH 7.4; anionic carboxylate drives membrane impermeability). logD7.4 and pKa not reported with a citable value (left blank). Actual payload is the AS269/amberstatin MMAF derivative, so MMAF values are a close proxy. CLINICAL (ESMO 2025 abstr 959P; tier D, conference abstract): China first-in-human Phase 1, advanced solid tumors, ARX305 0.24-4.2 mg/kg IV Q3W, Bayesian Optimal Interval dose-escalation; 41 patients enrolled Oct 2022-May 2025 (RCC 70.7%; also liver, ampulla of Vater, colorectal, nasopharyngeal, parotid). RP2D/MTD NOT yet determined (escalation ongoing) - so rp2d_dose left as "not determined" and n_rp2d blank; all reported rates are POOLED across the full 41-patient dose-escalation safety population (this single pooled cohort is the primary/headline cohort, tagged accordingly in line_context). CD70 by IHC: >=1% = positive, >=50% = high. SAFETY: TRAE 85.4%; grade>=3 39.0% (16/41); no grade 5. Ocular (priority; all grade 1-2, none >=G3): dry eye 46.3%, vision blurred 43.9%, keratopathy 41.5% - classic off-target MMAF-class corneal epitheliopathy triad (=> ocular_surface_subtype "Corneal (off-target)"; oae_any_pct 46.3 = max PT; oae_g3plus_pct 0 documented). Other common TRAEs: AST increased 58.5% (Hepatic), proteinuria 58.5% (renal), anaemia 43.9% (Heme), hyponatremia 34.1% (metabolic), fatigue 31.7% (general), thrombocytopenia 29.3% (Heme). NOT mapped to toxicity_rows because organ_system enum lacks a category: proteinuria (renal), hyponatremia (metabolic), fatigue (general/constitutional). No GI, pulmonary/ILD, peripheral-neuropathy, or infusion-reaction events reported among common TRAEs (consistent with low-PN MMAF profile) - left blank (= not in literature). Per-PT grade>=3 breakdown not available in accessible sources; toxicity_rows n values are back-calculated from reported % x N=41 (all clean integers). EFFICACY: ORR 26.1% (6/23) in CD70-positive evaluable; 42.9% (6/14) in high-CD70 RCC. SOURCE ACCESS NOTE: annalsofoncology.org fulltext and the Larvol mirror both returned HTTP 403; clinical figures were extracted from the indexed search abstract content (corroborated by the curated wave1 oae_hint), ADCdb, adcreview.com, and Ambrx preclinical disclosures. Tiers: composition/adcdb/payload_physchem/chemistry = C (ADCdb + PubChem-computed + company abstracts); dosing/ocular/toxicity = D (conference abstract).